Journal: Scientific Reports

Article Title: Screening DNA-targeted anticancer drug in vitro based on cancer cells DNA-templated silver nanoclusters

doi: 10.1038/s41598-019-45523-2

Figure Lengend Snippet: IC 50 of DNA-AgNCs-drug system against SMMC-7721 cells a .

Article Snippet: SMMC-7721 cells were obtained from Changsha & Information Industry Park (Liuyang National Economic & Technical Development Zone), The solutions of MIT (Rui Xin Pharmaceutical Co., Zhe Jiang, China), EPI, DAU and ADR (Hai Zheng Pharmaceutical Co., Zhe Jiang, China) were prepared in the conversation of 100.0 μg·mL −1 .

Techniques:

Journal: Scientific Reports

Article Title: Screening DNA-targeted anticancer drug in vitro based on cancer cells DNA-templated silver nanoclusters

doi: 10.1038/s41598-019-45523-2

Figure Lengend Snippet: Apoptosis rate of DNA-Ag NCs-drug system against SMMC-7721 cells β .

Article Snippet: SMMC-7721 cells were obtained from Changsha & Information Industry Park (Liuyang National Economic & Technical Development Zone), The solutions of MIT (Rui Xin Pharmaceutical Co., Zhe Jiang, China), EPI, DAU and ADR (Hai Zheng Pharmaceutical Co., Zhe Jiang, China) were prepared in the conversation of 100.0 μg·mL −1 .

Techniques: Negative Control

Journal: OncoTargets and therapy

Article Title: circ_0003418 Inhibits Tumorigenesis And Cisplatin Chemoresistance Through Wnt/β-Catenin Pathway In Hepatocellular Carcinoma

doi: 10.2147/OTT.S229507

Figure Lengend Snippet: circ_0003418 downregulation in HCC tissues and cells. Notes: ( A ) The expression profile of circ_0003418 in HCC tissues and adjacent noncancerous tissues were detected by qRT-PCR assays. ( B ) qRT-PCR was used to analyze the level of circ_0003418 in five HCC cell lines and one normal human hepatocyte cell line. ( C and D ) The knockdown efficiency of LV3-circ_0003418 on circ_0003418 in Huh-7 and Hep-3B cells was verified via qRT-PCR. ** P<0.01 and *** P<0.001 compared to control group. Abbreviations: HCC, hepatocellular carcinoma; NC, negative control.

Article Snippet: Human HCC cell lines (Hep-3B, Huh-7, Sk-hep-1, SMMC-7721 and PLC) and normal human hepatocyte line (HL-77O2) were purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Control, Negative Control

Journal: OncoTargets and therapy

Article Title: circ_0003418 Inhibits Tumorigenesis And Cisplatin Chemoresistance Through Wnt/β-Catenin Pathway In Hepatocellular Carcinoma

doi: 10.2147/OTT.S229507

Figure Lengend Snippet: circ_0003418 suppresses proliferation, migration, and invasion and promotes apoptosis in HCC cells. Notes: ( A and B ) CCK-8 assays were performed to measure the effect of silencing circ_0003418 on the proliferation in Huh-7 and Hep-3B cells. ( C–F ) Effect of silencing circ_0003418 on cell migration ( C and D ) and invasion ( E and F ) were analyzed by transwell migration and invasion assays, respectively. * P<0.05, ** P<0.01 and *** P<0.001 compared to control group. Abbreviations: CCK-8, cell counting kit 8; NC, negative control.

Article Snippet: Human HCC cell lines (Hep-3B, Huh-7, Sk-hep-1, SMMC-7721 and PLC) and normal human hepatocyte line (HL-77O2) were purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, CCK-8 Assay, Control, Cell Counting, Negative Control

Journal: OncoTargets and therapy

Article Title: circ_0003418 Inhibits Tumorigenesis And Cisplatin Chemoresistance Through Wnt/β-Catenin Pathway In Hepatocellular Carcinoma

doi: 10.2147/OTT.S229507

Figure Lengend Snippet: circ-0003418 sensitizes HCC cells to cisplatin in vitro. Notes: ( A and B ) Huh-7 and Hep-3B cells infected with LV3-NC or LV3-circ_0003418 were treated with different doses of cisplatin (1, 2, 4, 8, 16, 32, 64 and 128 mg/L) for 24 hrs, and then cell viability was determined by CCK-8 assays. ( C–F ) Huh-7 cells were treated with cisplatin (11.39 mg/L) for 24 hrs as well as Hep-3B cells were treated with cisplatin (20.18 mg/L) for 24 hrs, and then cell migration and invasion were detected by transwell migration ( C and D ) and invasion ( E and F ) assays, respectively. * P<0.05, ** P<0.01 and *** P<0.001 compared to control group. Abbreviation: NC, negative control.

Article Snippet: Human HCC cell lines (Hep-3B, Huh-7, Sk-hep-1, SMMC-7721 and PLC) and normal human hepatocyte line (HL-77O2) were purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: In Vitro, Infection, CCK-8 Assay, Migration, Control, Negative Control

Journal: OncoTargets and therapy

Article Title: circ_0003418 Inhibits Tumorigenesis And Cisplatin Chemoresistance Through Wnt/β-Catenin Pathway In Hepatocellular Carcinoma

doi: 10.2147/OTT.S229507

Figure Lengend Snippet: circ-0003418 enhances sensitivity of HCC cells to cisplatin in vivo. Female BALB/c nude mice were implanted subcutaneously with Huh-7 cells infected with LV3-NC or LV3-circ_0003418. Twelve days later, the LV3-NC tumor-bearing mice were treated with saline or cisplatin (5 mg/kg) by intraperitoneal injection twice a week up to 36 days posttreatment. The mice bearing LV3-circ_0003418 tumors received the same treatment. Notes: ( A ) Image of the tumors in the nude mice. ( B ) The tumors growth curve of xenograft model mice. ( C and D ) The weight and volume of the tumors in the nude mice. * P<0.05, ** P<0.01 and *** P<0.001 compared to control group. Abbreviation: NC, negative control.

Article Snippet: Human HCC cell lines (Hep-3B, Huh-7, Sk-hep-1, SMMC-7721 and PLC) and normal human hepatocyte line (HL-77O2) were purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: In Vivo, Infection, Saline, Injection, Control, Negative Control

Journal: OncoTargets and therapy

Article Title: circ_0003418 Inhibits Tumorigenesis And Cisplatin Chemoresistance Through Wnt/β-Catenin Pathway In Hepatocellular Carcinoma

doi: 10.2147/OTT.S229507

Figure Lengend Snippet: Silencing circ_0003418 induces cisplatin resistance of HCC cells through activating the Wnt/β-catenin pathway. Notes: ( A and B ) Huh-7 cells were treated with cisplatin (11.39 mg/L) for 24 hrs as well as Hep-3B cells were treated with cisplatin (20.18 mg/L) for 24 hrs, and then the protein levels of β-catenin and c-Myc in the Huh-7 and Hep-3B cells were detected by Western blotting. ( C and D ) The inhibition efficiency of ICG-001 was detected by Western blotting. ( E and F ) Cell proliferation assay showed that inhibition of Wnt/β-catenin pathway in cells infected with LV3-circ_0003418 inhibited cell proliferation. ( G and H ) Chemotherapy sensitivity assay showed that inhibition of Wnt/β-catenin pathway in cells infected with LV3-circ_0003418 enhanced sensitivity of HCC cells to cisplatin. * P<0.05, ** P<0.01 and *** P<0.001 compared to control group. Abbreviation: NC, negative control.

Article Snippet: Human HCC cell lines (Hep-3B, Huh-7, Sk-hep-1, SMMC-7721 and PLC) and normal human hepatocyte line (HL-77O2) were purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Western Blot, Inhibition, Proliferation Assay, Infection, Sensitive Assay, Control, Negative Control

Journal: Chinese Medical Journal

Article Title: USP25 promotes hepatocellular carcinoma progression by interacting with TRIM21 via the Wnt/β-catenin signaling pathway

doi: 10.1097/CM9.0000000000002714

Figure Lengend Snippet: USP25 is highly expressed in HCC and is correlated with poor prognosis. (A) The expression of USP25 in pan-carcinoma as determined with the TIMER database. (B) The expression of USP25 mRNA was analyzed in HCC tissues from TCGA and ICGC databases. (C) The protein expression levels of USP25 in a normal liver cell line and six HCC cell lines. (D) The expression of USP25 in different HCC cell lines in the CCLE database. (E) Kaplan–Meier survival analysis of survival in HCC patients grouped according to USP25 expression ( n = 370). (F) The promoter methylation level of USP25 in different grades of HCC according to the analysis of patient samples. Grade 1: well differentiated; Grade 2: moderately-differentiated; Grade 3: moderately and poorly differentiated; Grade 4: poorly differentiated. (G) The expression of USP25 in HCC patients based on nodal metastasis status. * P <0.05; † P <0.001; ‡ P <0.01, § P <0.0001. ACC: Adrenocortieal carcinoma; BLCA: Bladder urothelial carcinoma; BRCA:Breast invasive carcinoma; CESC: Cervical squamous cell carcinoma; CHOL: Cholangiocarcinoma; COAD: Colon adenocarcinoma; DLBC: Lymphoid neoplasm diffuse large B-cell lymphoma; ESCA: Esophageal carcinoma; GBM: Glioblastoma multiforme; HNSC: head and neck squamous cell carcinoma; KICH: Kidney chromophobe; KIRC: Kidney renal clear cell carcinoma; KIRP: Kidney renal papillary cell carcinoma; LAML: Acute myeloid leukemia; LGG: Brain lower grade glioma; LIHC: Liver hepatocellular carcinoma; LUAD: Lung adenocarcinoma; LUSC: Lung squamous cell carcinoma; MESO: Mesothelioma; OV: Ovarian serous cystadenocarcinoma; PAAD: Pancreatic adenocarcinoma; PCPG: Pheochromocytoma and paraganglioma; PRAD: Prostate adenocarcinoma; READ: Rectum adenocarcinoma; SARC: Sarcoma; SKCM: Skin cutaneous melanoma; STAD: Stomach adenocarcinoma; TGCT: Testicular germ cell tumors; THCA: Thyroid carcinoma; THYM: Thymoma; UCEC: Uterine corpus endometrial carcinoma; UCS: Uterine carcinosarcoma; UVM: Uveal melanoma; HCC: Hepatocellular carcinoma; ICGC: International Cancer Genome Consortium; TIMER: Tumor Immune Estimation Resource; USP25: Ubiquitin-specific peptidase 25; TCGA: The Cancer Genome Atlas; CCLE: Cancer Cell Line Encyclopedia; HR: Hazard ratio; L-O2: Normal hepatocyte cell line; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; N0, 1: Node0,1.

Article Snippet: Six human HCC cell lines (HepG2, HuH7, SMMC7721, Bel7402, MHCC97H, and PLC/PRF/5) and a normal hepatocyte cell line (L-O2) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Methylation, Ubiquitin Proteomics

Journal: Chinese Medical Journal

Article Title: USP25 promotes hepatocellular carcinoma progression by interacting with TRIM21 via the Wnt/β-catenin signaling pathway

doi: 10.1097/CM9.0000000000002714

Figure Lengend Snippet: USP25 promotes HCC cell proliferation in vitro. (A–C) HepG2 and MHCC97H cell lines with USP25 overexpression or knockdown were successfully established, as verified by analysis of protein and mRNA levels and fluorescence microscopy (Original magnification × 100). (D–F) Colony formation and CCK-8 assays were performed to detect cell proliferation. (G–J) DNA synthesis was measured by EdU IF staining (Original magnification × 200). * P <0.05; † P <0.001; ‡ P <0.01.CCK-8: Cell counting kit-8; HCC: Hepatocellular carcinoma; IF: Immunofluorescence; USP25: Ubiquitin-specific peptidase 25; EdU: 5-ethnyl-2-deoxyuridine; DAPI: 4′,6-Diamidino-2′-phenylindole; shUSP25: short hairpin Ubiquitin-specific peptidase 25; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: Six human HCC cell lines (HepG2, HuH7, SMMC7721, Bel7402, MHCC97H, and PLC/PRF/5) and a normal hepatocyte cell line (L-O2) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: In Vitro, Over Expression, Knockdown, Fluorescence, Microscopy, CCK-8 Assay, DNA Synthesis, Staining, Cell Counting, Immunofluorescence, Ubiquitin Proteomics

Journal: Chinese Medical Journal

Article Title: USP25 promotes hepatocellular carcinoma progression by interacting with TRIM21 via the Wnt/β-catenin signaling pathway

doi: 10.1097/CM9.0000000000002714

Figure Lengend Snippet: USP25 promotes HCC cell migration and invasion in vitro. (A,B) A Transwell invasion assay was performed to assess the invasiveness of HepG2 and MHCC97H cells (Original magnification × 100). (C,D) A transwell migration assay was performed to analyze the migratory ability of HepG2 and MHCC97H cells (Original magnification × 100). (E–H) The scratch assay results demonstrated the migratory ability of HepG2 and MHCC97H cells (Original magnification × 40). (I,J) The expression levels of a PCNA and invasion-related proteins (MMP2 and MMP9) were detected by Western blotting. * P <0.001; † P <0.01. HCC: Hepatocellular carcinoma; PCNA: Proliferation-related protein; USP25: Ubiquitin-specific peptidase 25; MMP2: Matrix metallopeptidase 2; MMP9: Matrix metallopeptidase 9; shUSP25: short hairpin Ubiquitin-specific peptidase 25; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: Six human HCC cell lines (HepG2, HuH7, SMMC7721, Bel7402, MHCC97H, and PLC/PRF/5) and a normal hepatocyte cell line (L-O2) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, In Vitro, Transwell Invasion Assay, Transwell Migration Assay, Wound Healing Assay, Expressing, Western Blot, Ubiquitin Proteomics

Journal: Chinese Medical Journal

Article Title: USP25 promotes hepatocellular carcinoma progression by interacting with TRIM21 via the Wnt/β-catenin signaling pathway

doi: 10.1097/CM9.0000000000002714

Figure Lengend Snippet: USP25 promotes HCC growth by activating the Wnt/β-catenin signaling pathway. (A) The expression of β-catenin was observed by IF staining (Original magnification × 200). (B) The mRNA expression of components in the Wnt/β-catenin signaling pathway was measured by qRT-PCR. (C) The expression of β-catenin was observed by Western blotting of nuclear-cytoplasmic fractionation. (D, E) Western blotting was used to detect the protein expression levels of Wnt/β-catenin pathway components and downstream factors. (F, G) Western blotting analysis after XAV939 treatment of HepG2 and MHCC97H cells. * P <0.001; † P <0.01; ‡ P <0.05, shUSP25 vs . Control; § P <0.05, shUSP25+XAV939 vs . Control. HCC: Hepatocellular carcinoma; IF: Immunofluorescence; qRT-PCR: Quantitative real-time polymerase chain reactio; USP25: Ubiquitin-specific peptidase 25.DAPI: 4′,6-Diamidino-2′-phenylindole; shUSP25: Short hairpin Ubiquitin-specific peptidase 25; PCNA: Proliferating cell nuclear antigen GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GSK3β: Glycogen synthase kinase 3 beta.

Article Snippet: Six human HCC cell lines (HepG2, HuH7, SMMC7721, Bel7402, MHCC97H, and PLC/PRF/5) and a normal hepatocyte cell line (L-O2) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Staining, Quantitative RT-PCR, Western Blot, Fractionation, Control, Immunofluorescence, Ubiquitin Proteomics

Journal: Oncogene

Article Title: The β-catenin/TCF-4-LINC01278-miR-1258-Smad2/3 axis promotes hepatocellular carcinoma metastasis

doi: 10.1038/s41388-020-1307-3

Figure Lengend Snippet: a The expression of miR-1258 in 20 paired E-HCC tissues and adjacent normal tissues. b Correlation of recurrence-free survival (RFS) and overall survival (OS) and miR-1258 expression by Kaplan–Meier analysis in TCGA datasets. c Correlation of RFS and OS and miR-1258 expression by Kaplan–Meier analysis in SYSUCC datasets. d The expression of miR-1258 in immortalized liver cells (THLE-3) and four HCC cell lines (SMMC-7721, Huh-7, HepG2, and Hep3B). e The wound-healing assay of HCC cells. f The invasion assay of HCC cells. g The numbers of lung metastasis. Experiments performed in triplications. miR-1258, HCC cells were transfected by miR-1258 expression vector. Anti-miR-1258, HCC cells were transfected by miR-1258 antisense plasmid. * P < 0.05.

Article Snippet: Four HCC cell lines (SMMC-7721, Huh-7, HepG2, and Hep3B), and immortalized human liver cells (THLE-3) (China Center for Type Culture Collection, Wuhan, China; American Type Culture Collection, Manassas, VA, USA) were grown in Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA, USA) with 10% newborn calf serum at 37 °C in a humidified atmosphere containing 5% CO 2 .

Techniques: Expressing, Wound Healing Assay, Invasion Assay, Transfection, Plasmid Preparation